Internship project Master 2 Recherche, starting period second semester of February 2018
Laboratory: Association Plateforme BioPark d’Archamps
Operating Manager: Philippe BULET; Team: MassOmics Services
Name and status of scientist in charge of the project: Philippe BULET, Dr CNRS, HDR
Plateforme BioPark d’Archamps, Bâtiment le Forum, Archamps Technopole, 74160 Archamps
e-mail: [email protected], [email protected]
Objectives: This project has for main objective to image by MALDI-MSI the different anatomical compartments of two/three pollinators (honeybee, solitary bee, bumble bee) subjected to individual or multifactorial stressors (entomopathogenic bacteria and parasites, microsporidia, mites) and to deliver images of specific markers regulated by the stressors. We will start with honeybee (samples available all the year) while the selection of the two pollinator models and of two stressors will be defined at the beginning of the beekeeping season (early March 2018) in agreement with our consultant. We have
Abstract: The complexity of organs is defined not only by the amount of molecular events they contain but also by the spatial and temporal distribution of molecules in response to environmental stimuli. For two decades, the advent of analytical strategies applied to life sciences has given rise to a molecular imaging technology based on matrix assisted laser desorption mass spectrometry (MALDI-MSI) whose versatility allows mapping at any point of a biological matrix, various compounds ranging from metabolites to proteins. Unlike conventional imaging methods used in pharmacokinetics / pharmacodynamics, MSI allows mapping biomolecules in tissues without using immunostaining or radiolabelled probes. MSI will be applied to study in the honey bee the molecular disturbance in response to different stressors or infectious agents. The objective is to achieve a histomolecular atlas of a bee by innovative MALDI-MSI. Additional data will be acquired using more conventional proteomics approaches (Bottom-up, Top-down) on dissected targeted tissues.
Methods: Mass spectrometry imaging (MALDI-MSI) and Bottom-up/Top-down proteomics (nano-LC and High resolution MS) will be at the center of this project. Complementary methods such as conventional histology and computational imaging will be contributed to reach the objectives. Different methods in microbiology and histology will be used to conduct this study.
Publications of the team related to MALDI-MSI and proteomics:
1. Pisani C, Voisin S, Arafah K, Durand P, Perrard MH, Guichaoua MR, Bulet P, Prat O. Ex vivo assessment of testicular toxicity induced by carbendazim and iprodione, alone or in a mixture. ALTEX. 2016;33(4):393-413. doi: 10.14573/altex.1601253
2. Bulet P & Arafah K (284 pages, 2014) Protéomique conventionnelle (pp 95-99) et Protéomique
3. et imagerie MALDI (pp 99-103) dans Méthodes en Immunologie, Des principes aux bonnes
4. applications. Collège des enseignants d’immunologie (Assim) Coordinateurs de l’ouvrage : Marie-
5. Christine Béné, Christian Drouet, Sylvain Fisson et Estelle Seillès. Editeur Elsevier-Masson.
6. Arafah K. & Bulet P., Book Chapter in “Biotechnology en Santé-Tome 1 Analyse et Diagnostic”, Mass Spectrometry Imaging, November 2015
7. Arafah K, Longuespée R, Desmons A, Kerdraon O, Fournier I, Salzet M. Lipidomics for clinical diagnosis: Dye-Assisted Laser Desorption/Ionization (DALDI) method for lipids detection in MALDI mass spectrometry imaging.OMICS. 2014 Aug;18(8)
8. Herren JK, Paredes JC, Schüpfer F, Arafah K, Bulet P, Lemaitre B. Insect endosymbiont proliferation is limited by lipid availability. Elife. 2014 Jul 15;3:e02964. doi: 10.7554/eLife.02964
Requested domains of expertise (few keywords): Biochemistry, mass spectrometry, proteomics, mass spectrometry imaging, histoproteomy, histology